1. Intracellular recordings were made from inner and outer hair cells in the basal turn of the guinea-pig cochlea. The resting membrane potentials of the inner hair cells are more positive than -50 mV while those of outer hair cells are usually more negative than -70 mV. 2. At low frequencies the receptor potentials of inner hair cells are predominantly depolarizing while those from outer hair cells are hyperpolarizing at low and moderate sound pressure (e.g. less than 90 dB re 2 X 10(-5) Pa at 600 Hz). The potentials then become predominantly depolarizing at high sound pressure. 3. The asymmetry of the inner and outer hair cell receptor potentials are manifested instantaneously except at high stimulus levels when the depolarizing responses of outer hair cells take several cycles to develop. 4. At the offset of intense tones outer hair cell membrane potentials remain depolarized by 1-2 mV above their resting value and return to normal over a period depending on the level and duration of the tone. 5. In response to tones above about 2 kHz and at levels below about 90 dB the wave forms of outer hair cell receptor potentials are virtually symmetrical without measurable d.c. components. In response to tones close to their best frequencies (16-21 kHz), inner hair cells in the basal turn generate large depolarizing (d.c.) receptor potentials while outer hair cells from this region of the cochlea do not generate significant voltage responses. 6. Frequency tuning curves were derived for inner and outer hair cells from the amplitude-intensity relationships of their d.c. and phasic (a.c.) receptor potentials respectively. When the latter were compensated for the low-pass characteristics of the recording system and the hair cell time constant, the frequency selectivity of inner and outer hair cells are similar. 7. The response properties of inner and outer hair cells in the basal turn of the guinea-pig cochlea are discussed in relation to their proposed roles in mechano-electric transduction.
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机译：1.在豚鼠耳蜗基底转弯处，从内部和外部毛细胞进行细胞内记录。内部毛细胞的静息膜电位高于-50 mV，而外部毛细胞的静息膜电位通常高于-70 mV。 2.在低频下，内部毛细胞的受体电势主要去极化，而来自外部毛细胞的受体电势则在低和中等声压（例如，在600 Hz时小于90 dB re 2 X 10（-5）Pa）超极化。然后，在高声压下，电势主要变为去极化。 3.当在高刺激水平下，当外毛细胞的去极化反应需要几个周期发展时，内和外毛细胞受体电位的不对称即刻表现出来。 4.在强烈的音调抵消下，外部毛细胞膜电位仍会在其静止值以上1-2 mV消极化，并在一段时间内恢复正常，具体取决于音调的级别和持续时间。 5.响应高于约2kHz且低于约90dB的音调，外部毛细胞受体电位的波形实际上是对称的，而没有可测量的直流。组件。为了响应接近其最佳频率（16-21 kHz）的音调，基匝内的内部毛细胞会产生较大的去极化（d.c.）受体电位，而来自耳蜗此区域的外部毛细胞不会产生明显的电压响应。 6.从内部和外部毛细胞的直流振幅强度关系中得出频率调谐曲线。和相（交流）受体电势。当补偿后者的记录系统的低通特性和毛细胞时间常数时，内部和外部毛细胞的频率选择性相似。 7.讨论了豚鼠耳蜗基回合中的内毛细胞和外毛细胞的响应特性及其在机电传导中的拟议作用。
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